 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Influences of Anthraquinone Extraction Techniques from Morinda sp. on Extraction Efficiency) ผู้เขียน: Wongwat Temiyaputra, Thidarat Suebsanga, Kesinee Yajom, Sirilak Piyaworanon, Noppol Leksawasdi สื่อสิ่งพิมพ์:pdf AbstractThe comparison of anthraquinone levels in root, stem, bark and leaf of Morinda sp. were investigated by HPLC with Alltima? C8 column using 32%(v/v) acetonitrile and 0.5% (v/v) acetic acid as mobile phase at 40?C for 20 min. The roots contained the highest specific concentration of anthraquinone at 103.2 ? 4.8 mg/g dried mass, followed by stem (45.3 ? 6.3 mg/g), bark (6.90 ? 0.3 mg/ g) and leaf (1.20 ? 0.6 mg/g). Four extraction techniques were employed to extract anthraquinone from the roots of Morinda sp and the anthraquinone content was analyzed using spectrophotometer. Soxhlet apparatus was utilized in the first technique with 50% (v/v) methanol as an extraction solvent. It was found that 14.6 ? 1.0 mg/g dried root powder was extracted with the expenses of 3.97 ? 0.17 Baht/mg anthraquinone. This was compared to the second extraction method at room temperature with 80%(v/v) acetone and 50%(v/v) ethanol that led to the highest concentration of extracted anthraquinone at 38.9 ? 1.6 and 27.0 ? 6.9 mg/g with the accompanied cost of 2.94 ? 0.12 and 1.42 ? 0.36 Baht/mg, respectively. The third extraction procedure was carried out in the pressurised steamer at 1 bar and 100?C for 5 min with 80% (v/v) ethanol. The highest level of extracted anthraquinone was 95.3 ? 0.6 mg/g at the cost of 4.28 ? 0.03 Baht/mg. The last method of extraction was performed in the closed-circuit solid-liquid extraction unit at room temperature. Although the extraction cost was relatively small (1.50 ? 0.08 Baht/mg), the specific concentration obtained was not high (only 2.27 ? 0.11 mg/g). |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Anthraquinone Extraction from Morinda sp. Root Powder Under Steam Pressurized Conditions) ผู้เขียน:Taweyot Kunyotying, Noppol Leksawasdi สื่อสิ่งพิมพ์:pdf AbstractThree types of containers and six strategies to control the evaporation of 80%v/v ethanol for extraction of anthraquinone from dried Morinda sp. root powder in a pressurized steamer at 20 psi for 30 min were investigated. These were the most extreme conditions possible in the system investigated. A tightly sealed 231 ml jam jar with a metal screw cap (diameter 5.4 cm, height 10.1 cm) was chosen as the most appropriate container with the ability to withhold 65.0 ? 5.6% of the original liquid volume after pressurization with saturated steam. The optimal pressure level and time for pressurized anthraquinone extraction in the jar containing 80%v/v ethanol was 5 psi for 5 min with the corresponding level of extracted anthraquinone being 30.9 ? 0.5 mg/g dried root powder, with a minimal extraction cost of merely 2.65 ? 0.04 baht/mg. Further experiments on the effect of varying the ethanol concentration revealed that 65%v/v ethanol achieved the highest extracted anthraquinone level of 38.4 ? 0.7 mg/g with a corresponding extraction cost of only 2.01 ? 0.04 baht/mg. The problem of reproducibility and the existence of outliers encountered previously with pressurized steam extraction were resolved and eliminated with the use of the 231 ml jam jar. |